LOCUS       40924_24653        5399 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pHIS3K DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pHIS3K
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5399)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5399)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    242..808
                     /pseudo
                     /gene="his3"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             242..808
                     /pseudo
                     /codon_start=1
                     /gene="his3"
                     /label=his3
                     /note="NotI site is created at the middle of this fragment 
                     (487-494)."
                     /translation="VLAAMKLLTP*FAFLVFLEKIRF*CVHLRTECIRSLQKSTMLRLL
                     RFSLNQISI*TSMLFAKHFLKIVPSKSFLLVPLVIQQRPLLNSRILRRFWSTLHGMESL
                     SSMRPILISLLPICLP*LLSMNIQTLLFAKPFRNLSV*QEFGKLI*AFKH*YYFFTNYD
                     V*YRILFDK*TYCYHYELIKGSL*H"
     gene            242..808
                     /pseudo
                     /gene="his3"
                     /label=his3
     gene            852..2208
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     complement(2259..2275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2488..2943
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3225..3329
                     /label=AmpR promoter
     CDS             3330..4187
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4361..4949
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5237..5258
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5273..5303
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5311..5327
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5335..5351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtgttggca gcgatgaaat tattgactcc ttaattcgca tttcttgtat tcctggaaaa
      301 gataagattt tgatgtgtcc accttcgtac ggaatgtata cggtctctgc aaaaatcaac
      361 gatgttgagg ttgttaaggt tctccttgaa ccagatttca atttgaacgt cgatgctatt
      421 tgcgaaacac tttctaaaga tagtgccatc aaagtctttt ttgcttgttc ccctggtaat
      481 ccaacagcgg ccgctcttaa actcgaggat attaagaaga ttttggagca ccctacatgg
      541 aatggaatcg ttgtcgtcga tgaggcctat attgatttct ctgctcccga tatgtctgcc
      601 ttaactcttg tcaatgaata tccaaacctt gctgtttgcc aaaccctttc gaaatctttc
      661 ggtctagcag gaattcggta agttaatcta agcttttaag cattaatatt atttctttac
      721 taactatgat gtttagtatc ggattttgtt tgacaagtaa acctattgct accattatga
      781 actcattaaa ggctccttat aacattagat ctgtttagct tgcctcgtcc ccgccgggtc
      841 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      901 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      961 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
     1021 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
     1081 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
     1141 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg
     1201 taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1261 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
     1321 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1381 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1441 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa
     1501 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1561 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1621 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     1681 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     1741 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1801 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     1861 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     1921 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1981 tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag
     2041 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
     2101 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
     2161 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     2221 tactaacgcc gccatccagt ttaaacgagc tcgaattcac tggccgtcgt tttacaacgt
     2281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2401 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2461 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     2521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2701 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2821 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2881 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     2941 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3001 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     3061 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     3121 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3181 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3841 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3901 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3961 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4021 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4081 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4141 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4201 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4261 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4501 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5101 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     5161 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     5221 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     5281 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     5341 acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct agaggatcc
//
